BIOTECNOLOGIE DEI PRODOTTI BIOLOGICAMENTE ATTIVI E MICROBICIModule BIOTECNOLOGIE DEI PRODOTTI BIOLOGICAMENTE ATTIVI
Academic Year 2023/2024 - Teacher: GIUSEPPE MALFAExpected Learning Outcomes
Provide the student with the ability to:
• Autonomously apply the acquired knowledge to the solution of problems related to pharmaceutical biology.
• Know the specific methodologies in the study of biologically active products obtainable from renewable sources through biotechnological processes.
• Describe the fundamental biological processes of the plant cell and plants to understand the specific methodologies in the study of biologically active products
• Acquire skills in the botanical-pharmaceutical field and applications aimed at technical-professional fields aimed at the production and control of biotechnological drugs, hormones and vaccines, and bioactive natural products.
Course Structure
Frontal lessons with the aid of slides, and videos from the internet.
Problem-based learning.
If the teaching is given in mixed or distance learning mode, the necessary changes may be introduced concerning what was previously stated, to respect the program envisaged and reported in the syllabus.
Information for students with disabilities and/or DSA
To guarantee equal opportunities and compliance with the laws in force, interested students can request a personal interview to plan any compensatory and/or dispensatory measures, based on the educational objectives and specific needs. It is also possible to contact the CInAP contact person (Centre for Active and Participatory Integration - Services for Disabilities and/or DSA) of the Department of Biomedical and Biotechnological Sciences.
Required Prerequisites
Attendance of Lessons
Attendance is compulsory as required by the teaching regulations of the Degree Course (http://www.biometec.unict.it/it/corsi/l-2/regolamento-didattico)
The Biotechnology of Biologically Active Products course will be divided into frontal lessons (3 credits) and group laboratory exercises dedicated to the extraction, identification, and quality control techniques of pharmacological plant metabolites for the production of biotechnologically active plant products/ingredients. active (3 CFU) shared and cooperative activities, during which students will be invited to participate in feedback evaluation activities.
Detailed Course Content
Introduction to the study of biotechnology of biologically active products.
Specific cytological structures of plants. Meristematic and differentiated tissues. Parenchymatic, integumental, mechanical, vascular and secretory systems.
Structure of plants: root, stem, and leaves.
Reproduction of plants: asexual reproduction and sexual reproduction: flower, inflorescences, fruit, and seed.
Plant and pharmaceutical classification.
Vegetable raw materials. Procurement of Medicinal Plants.
Vegetable matrix: classification and factors influencing the content of active ingredients. Wild and cultivated medicinal plants.
Primary metabolites of pharmaceutical interest: carbohydrates, fats, proteins.
Secondary metabolites of pharmaceutical interest. Biosynthesis and characteristics. Phenolic compounds, terpenoids, alkaloids.
Elements of: Preparation, extraction, and identification of the active principles. Crushing, shredding, and pulverizing. Squeezing and compression. Extraction with supercritical fluids. Distillation. decoction. Enfleurage. Maceration. For breakfast. Isolation of active ingredients. Qualitative and quantitative control of a drug. Dosage of active ingredients.
Renewable sources of medicines: algae, fungi, lichens, gymnosperms, and angiosperms.
Biotechnology of medicinal plants. Plant biotechnologies as sources of products of pharmaceutical interest. Agro-biotechnology. Preparation of in vitro cultures. Biotechnology in the cultivation of medicinal plants. In vitro cultures of medicinal interest. Biotechnological production of secondary metabolites. Biotransformations.
Pharmacist plants for the production of hormones, vaccines, and natural and bioactive products.
Elements of cultivation and transformation of medicinal plants.
Elements of quality control of medicinal plants.
Healthy products of vegetable origin.
The program is divided into three parts based on a temporal scan of the topics covered.
FIRST PART 1 CFU LT – 7 h
Introduction to the study of Pharmaceutical Biology.
Specific cytological structures of plants. Meristematic and differentiated tissues. Parenchymatic, integumental, mechanical, vascular and secretory systems.
Structure of plants: root, stem, and leaves.
Reproduction of plants; flower, inflorescence, fruit, and seed.
Plant and pharmaceutical classification.
Vegetable raw materials. Procurement of Medicinal Plants.
Vegetable matrix: classification and factors influencing the content of active ingredients. Wild and cultivated medicinal plants.
Primary metabolites of pharmaceutical interest: carbohydrates, fats, proteins.
Secondary metabolites of pharmaceutical interest. Biosynthesis and characteristics. Phenolic compounds, terpenoids, alkaloids.
SECOND PART 3 ECTS ELG (group laboratory exercise) - 36 h
Preparation of plant matrices; extraction techniques and identification of active ingredients.
Extraction techniques Crushing, shredding, and pulverizing. Squeezing and compression. Extraction with supercritical fluids. Distillation. decoction. Enfleurage. Maceration. For breakfast. Isolation of active ingredients. Qualitative and quantitative control of metabolites of pharmaceutical plants for the production of biotechnologically active plant products/ingredients. Theoretical-practical checks will be proposed at the end of each group exercise.
THIRD PART 2 CFU LT - 14 h
Renewable sources of medicines: algae, fungi, lichens, gymnosperms, and angiosperms.
Biotechnology of medicinal plants. Plant biotechnologies as sources of products of pharmaceutical interest. Agro-biotechnology. Preparation of in vitro cultures. Biotechnology in the cultivation of medicinal plants. In vitro cultures of medicinal interest. Biotechnological production of secondary metabolites. Biotransformations.
Pharmacist plants for the production of hormones, vaccines and natural and bioactive products.
Elements of cultivation and transformation of medicinal plants.
Elements of quality control of medicinal plants.
Healthy products of vegetable origin.
Textbook Information
1. Poli F. - Biologia Farmaceutica - II Ed. - Pearson
2019
2. Maugini E., Maleci Bini L., Mariotti Lippi M. - Manuale di Botanica
Farmaceutica IX Ed. – PICCIN 2014
3. Solomon, Berg, Martin -Struttura e processi vitali nelle piante- Ed. Edises
4. Bruni A., -Farmacognosia generale e applicata- ed. PICCIN
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Introduction to the Study of Pharmaceutical Biology Textbook 1: chs.1, 2 | 1. Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 2 | Specific cytological structures of plants. Textbook 2: ch. 4 | Maugini E., Maleci Bini L., Mariotti Lippi M. - Manuale di Botanica Farmaceutica IX Ed. – PICCIN |
| 3 | Meristematic and differentiated tissues. Textbook 2: ch. 4 | Maugini E., Maleci Bini L., Mariotti Lippi M. - Manuale di Botanica Farmaceutica IX Ed. – PICCIN |
| 4 | Parenchymatic, integumental, mechanical, vascular and secretory systems. Textbook 1 ch. 3; Textbook 3; ch 33 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 5 | Structure of plants: root, stem, and leaves. Textbook 1: chs. 4, 5 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 6 | Reproduction of plants: asexual reproduction and sexual reproduction: flower, inflorescences, fruit, and seed. Textbook 2: chs. 5, 6, 8 | Maugini E., Maleci Bini L., Mariotti Lippi M. - Manuale di Botanica Farmaceutica IX Ed. – PICCIN |
| 7 | Plant and pharmaceutical classification. Vegetable raw materials. Procurement of Medicinal Plants. Herbal drug: classification and factors influencing the content of active principles. Wild and cultivated medicinal plants. Textbook 4: chs. 5, 6 | Bruni A., -Farmacognosia generale e applicata- ed. PICCIN |
| 8 | Primary metabolites of pharmaceutical interest: carbohydrates, fats, proteins. Textbook 1; ch.7 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 9 | Secondary metabolites of pharmaceutical interest. Biosynthesis and characteristics. Phenolic compounds, terpenoids, alkaloids. Textbook 4: chs. 5, 6 | Bruni A., -Farmacognosia generale e applicata- ed. PICCIN |
| 10 | Crushing, shredding, and pulverizing. Squeezing and compression. Extraction with supercritical fluids. Distillation. decoction. Enfleurage. Maceration. For breakfast. Isolation of active ingredients. Qualitative and quantitative control of a drug. Dosage of active ingredients. Textbook 4: ch. 5 | Bruni A., -Farmacognosia generale e applicata- ed. PICCIN |
| 11 | Preparation, extraction, and identification of the active ingredients. Textbook 1; ch.10 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 12 | Renewable sources of medicines: algae, fungi, lichens, gymnosperms, and angiosperms. Textbook 1; chs.8-9-10-11 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 13 | Biotechnology of medicinal plants. Plant biotechnologies as sources of products of pharmaceutical interest. Agro-biotechnology. Preparation of in vitro cultures. Biotechnology in the cultivation of medicinal plants. In vitro cultures of medicinal interest. Textbook 1; chs. 7.8 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
| 14 | Biotechnological production of secondary metabolites. Biotransformations. Pharmacist plants for the production of hormones, vaccines, and natural and bioactive products. Elements of cultivation and transformation of medicinal plants. Control elements Textbook 1; chs. 9, 10 | Poli F. - Biologia Farmaceutica - II Ed. - Pearson 2019 |
Learning Assessment
Learning Assessment Procedures
Examples of frequently asked questions and / or exercises
1. Describe the morphology and classification of the plant body
2. Describe the morphology of meristematic tissues
4. Characteristics and function of primary and secondary metabolites
5. Biotechnology of medicinal plants.
6. Pharmacist plants for the production of hormones, vaccines and natural and bioactive products.