BASIC APPLIED MOLECULAR MICROBIOLOGY AND APPLIED TO MEDICINEModule MICROBIOLOGIA MOLECOLARE APPLICATA ALLA MEDICINA
Academic Year 2024/2025 - Teacher: Stefania STEFANIExpected Learning Outcomes
With the course of Medicine applied molecular microbiology students acquire fundamental knowledge relating to the morphology and functions of infectious agents of medical and biotechnological interest (viruses, bacteria, fungi and parasites), with particular attention to the structural characteristics, to the molecular features, to microorganism-host interaction, to replicative processes, to pathogenetic mechanisms and the transmission routes of pathogenic infectious agents, throughout the use of different models. Furthermore the course deals with antimicrobial agents and resistance, the use of monoclonal antibodies and vaccines.
Course Structure
1) The infectious process, etiological agents and normal microbiota
2) Principles of laboratory diagnosis of infectious diseases
3) Emergence and global spread of infections
4) Pathogenesis of viral infections and some insights into pathogenic viruses (chosen on the basis of their morphological and pathogenetic diversity)
5) Pathogenesis of bacterial infections and some insights into pathogenic bacteria
6) Pathogenesis of fungal infections: the Candida model
7) Antibacterial agents and resistance-
8) Prophylaxis, immunity, monoclonal antibodies and vaccines
Detailed Course Content
The microbial world, human microbiota, its role in health and disease conditions, infectious diseases, epidemiology, pathogenesis, immunity.
Samples, microscopy and staining, isolation and identification of bacteria, nutrient media; virus isolation and identification; immunological systems; antibody detection; nucleic acid analysis; probes.
Sources, infection and disease, incubation and transmission routes, epidemics and their control.
Characteristics of viruses, pathogenesis of viral infections, antiviral drugs and resistance
Characteristics of bacteria, pathogenesis of bacterial infections, some syndromic examples such as microorganisms associated with biofilm-related infections, bacteremia and sepsis from S. aureus, Neisseriae, Enterobacterales
Pathogenesis of fungal infections: the Candida model
Antibacterial agents and resistance-
Prophylaxis, immunity, monoclonal antibodies and vaccines
Textbook Information
One of these texts of your choice:
Medical Microbiology, Antonelli, Clementi, Pozzi, Rossolini, Ambrosiana Publishing House
Medical Microbiology, Murray, Rosenthal, Pfaller, Edra s.p.a.
Medical Microbiology, Sherris EMSI 2017
Articles will be provided by the teacher
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Tassonomia ed identificazione, interazione ospite-parassita, meccanismi molecolari dell'azione patogena | Deho'-Galli Biologia dei microrganismi - CEA editore |
| 2 | Analisi genomica, ricostruzxione evolutiva, applicazioni | pdf del docente |
| 3 | Agenti terapeutici, antibiotici e bersagli molecolari | pdf del docente |
| 4 | Cosa ci dice la genomica microbica | Deho'-Galli Biologia dei microrganismi - CEA editore |
| 5 | Produzione di batteriocine, regolazioni RNA e trascrittomica | Deho'-Galli Biologia dei microrganismi - CEA editore |
| 6 | Biofilm ed implicazioni | Deho'-Galli Biologia dei microrganismi - CEA editore |
| 7 | Vaccini tradizionali e ricombinanti | pdf del docente |
| 8 | applicazioni biotecnologiche in ambito biomedico | Glick et al - Molecula Biotechnology ASM USA |
| 9 | Il mondo microbico, microbiota umano, suo ruolo in condizioni di salute e malattia, le malattie infettive, epidemiologia, patogenesi, immunità. | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |
| 10 | Campione, microscopia e colorazioni, isolamento ed identificazione dei batteri, terreni nutritivi; isolamento ed identificazione dei virus; sistemi immunologici; rilevamento anticorpi; analisi acidi nucleici; sonde | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |
| 11 | Fonti, infezione e malattia, incubazione e vie di trasmissione, epidemie e loro controllo | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |
| 12 | Caratteristiche dei virus, patogenesi delle infezioni virali, farmaci antivirali e resistenze | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |
| 13 | Patogenesi delle infezioni fungine: il modello Candida | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |
| 14 | Agenti antibatterici e resistenze - | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |
| 15 | Profilassi, immunità, anticorpi monoclonali e vaccini | Testo a scelta tra: Microbiologia Medica, Antonelli, Clementi, Pozzi, Rossolini, Casa Editrice Ambrosiana Microbiologia Medica, Murray, Rosenthal, Pfaller, Edra s.p.a. Microbiologia Medica, Sherris EMSI 2017 Dispense ed arti |